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Pap Test
Special Stains


Frozen Section Specimens

Specimens for frozen sections should be sent immediately to Histology. Do not use formalin. Label specimen with Patient name, date of birth and specimen site/source. Complete tissue requisition AH80006(pdf) and include the following information.
  1. Legal first and last name
  2. Date of Birth
  3. Medical Center identification number or unique identifier
  4. First initial and last name of person submitting the specimen
  5. Date and time of collection
  6. First and last name of surgeon
  7. Specimen type
  8. Date taken to Histology
Note: Breast needle localized specimens will be taken to Radiology for films and then returned to Pathology for the frozen section.

Tissue Specimens

Specimens should be covered with formalin solution (except for the specimens listed in TABLE A) at a ratio of 1:10 (specimen to formalin). Containers should be hard plastic with lids that can be closed securely. Label the container with the patient's name, medical record number or other unique identifier and specimen site/source. Fill out requisition form AH080006 (pdf) for hospital inpatients/outpatients, or requisition AH080008 (pdf)for clinic s. Include the following information;
  1. Legal first and last name
  2. Date of Birth
  3. Medical Center identification number or unique identifier
  4. First initial and last name of person submitting the specimen
  5. Date and time of collection
  6. Time specimen was placed in formalin
  7. First and last name of surgeon
  8. Specimen type
  9. Date taken to Histology
  10. Specific requests such as margins requested, marking denoting positioning such as in conization
  11. Pertinent clinical data including clinical history.
Place specimen in Biohazard bag and requisition in the outside pocket for delivery to the laboratory.

Table A:

Do not place the following tissue specimens in formalin:
  1. Frozen sections
  2. Chromosome analysis (Cytogenetics)
  3. Cytology
  4. Tissue Cultures
  5. Microbiology
  6. Electron Microscopy
  7. Flow Cytometry
  8. Chemo sensitivity testing
  9. Muscle biopsies
  10. Nerve biopsies
  11. Kidney biopsies


  1. Legal first and last name
  2. Date of birth
  3. Medical center identification number or unique identifier
  4. Patient location
  5. Ordering physician
  6. First initial, last name of person collecting information
  7. Date and time of collection
  8. Pertinent clinical data including clinical history
  9. Label specimen container with patient name, date of birth, time and date of collection and specimen type.
The CYTOLOGY COLLECTION GUIDE includes the following:
  • BAL - Bronchoalveolar lavage
  • Body Fluids (effusion, paracentesis, pleural, thoracentesis, ascites)
  • Breast Nipple Discharge
  • Cerebral Spinal Fluid (CSF)
  • Fine needle biopsy
  • Gastric / Esophageal, Small intestine, pancratic and billiary brushings and/or washings
  • PAP smear (conventional slide method)
  • PAP smear ( liquid)
  • Broom Collection
  • Brush / Spatula collection
  • Respiratory specimens (sputum)
  • Respiratory specimens (bronchial brushings and washings)
  • Tzank smears (Herpes Virus)
  • Urine, bladder washings, Urethral washing, renal washings

    Pap Test

    Collection Instructions

    Thin Prep collection instructions - Broom
    Thin Prep collection instructions - Brush / Spatula
    GC/Chlamydia Molecular Testing Collection Flow Chart 

    • should not use vaginal medication, vaginal contraceptives, or douches 48 hours prior to exam
    • sample should not be taken during menses as excessive amounts of blood may compromise results.
    • Lubricant jellies should not be used to lubricate the speculum. Excessive amounts of jelly may compromise results
    • If a lubricant is necessary, use one of the following brands:
    1. KY Jelly
    2. Surgilube
    3. Astroglide
    4. Crystelle
    5. HR Lubricating Jelly
    • Excess cervical mucus is essentially devoid of meaningful cellular material
    • Please remove excess mucus before taking a sample for testing
    Collection Devices
    • Spatula - rinse by swirling in the vial 10 times
    • Brush - rinse brush in the solution 10 times while pushing against the vial wall.
    • Broom - rinse by pushing the broom into the bottom of the vial 10 times
    • Discard all collection devices after rinsing. Do not leave collection device in the Thin Prep liquid medium.

    Special Stains

    There are many special stains and antibodies stains available. To order any of these stains, please contact a pathologist at (402) 717-2875.

    Alegent Antibody List


    CPT Code: 88313
    1. 1.0pH Alcian Blue
    2. 2.5pH Alcian Blue
    3. 2.5pH Alcian Blue/H&E
    4. 2.5pH Alcian Blue/PAS
    5. Congo Red
    6. Crystal Violet
    7. Thioflavine T
    8. Mucicarmine
    9. PAS


    CPT Code: 88312
    1. Giemsa (also for Mast cells)
    2. Gram, Hunker-Twort
    3. AFB, Ziehl-Neelson
    4. Ag Fungus, GMS
    5. PAS Fungus
    6. Pneumo, GMS

    Connective Tissue

    CPT Code: 88313
    1. Reticulum
    2. Trichrome, Masson's
    3. Elastic, Verhoeff's


    CPT Code: 88313
    1. Oil Red O

    Pigments & Minerals

    CPT Code: 88313
    1. Iron, Mallory's
    2. Urate Crystals
    3. Bile

    Special Cells

    CPT Code: 88313
    1. Mast Cells, Toluidine Blue